Bradford’s assay is a rapid and accurate method for estimation of protein concentration. When compared to Lowry’s Method, it is subjected to less interference by common reagents and non-protein components of biological sample. The assay relies on the binding of the dye Coomassie blue G250 to protein. The quantity of protein can be estimated by determining the amount of dye in the blue ionic form. This is usually achieved by measuring the absorbance of the solution at 595 nm or 625 nm. The kit contains BSA as the protein standard, using which standard curves will be constructed. This curve will then be used to estimate the concentration of protein in test samples supplied.
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