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Forensic Services
The Lowry’s method of Protein estimation, discovered by Sir Folin Lowry is sensitive down to about 10 μg/ml and is probably the most widely used protein assay despite its being only a relative method , subject to interference from Tris buffer, EDTA, nonionic and cationic detergents, carbohydrate, lipids and some salts. The phenolic group of tyrosine and trytophan residues (amino acid) in a protein will produce a blue purple color complex, with maximum absorption in the region of 660 nm wavelength, with Folin- Ciocalteau reagent. Thus the intensity of color depends on the amount of these aromatic amino acids present and will thus vary for different proteins. Most proteins estimation techniques use Bovin Serum Albumin (BSA) universally as a standard protein, because of its low cost, high purity and ready availability. The method is The incubation time is very critical for a reproducible assay. The reaction is also dependent on pH and a working range of pH 9 to 10.5 is essential.
